لایب سان

Preface ................................................................................................. vii 1. Cell Encapsulation: Generalities, Methods, Applications and Bioartificial Pancreas Case Study ..................................................... 1 Gabriela Grigorescu and David Hunkeler Introduction .......................................................................................... 1 Immunoisolation ................................................................................... 4 Cell Delivery ......................................................................................... 5 Microencapsulation ............................................................................... 7 Bioartificial Organs ............................................................................... 7 Case Study: Insulin Production Systems .............................................. 10 Socio-Political Considerations ............................................................. 13 Conclusions......................................................................................... 14 2. Synthetic and Semisynthetic Polymers as Vehicles for In Vitro Gene Delivery into Cultured Mammalian Cells ................ 19 Martin Jordan Introduction: Impact of Molecular Biology ......................................... 19 Areas in Need of Efficient Gene Delivery ............................................ 20 The DNA Molecule ............................................................................ 21 Barriers to Efficient Gene Transfer ...................................................... 21 Conclusions......................................................................................... 35 Definitions .......................................................................................... 36 Structures of Frequently Used Molecules ............................................. 37 3. Affinity Precipitation: Stimulus-Responsive Polymers for Bioseparation .................................................................................. 40 Ruth Freitag Introduction ........................................................................................ 40 The Role of Affinity Separations in Product Isolation.......................... 41 The Principle and Application of Affinity Precipitation ....................... 43 Smart Polymers for Affinity Precipitation ............................................ 48 Thermosensitive AML ......................................................................... 50 Introduction of the Affinity Mediator ................................................. 53 Conclusions and Outlook.................................................................... 54 4. Synthetic Displacers for Preparative Biochromatography ..................... 58 Ruth Freitag and Christine Wandrey Introduction ........................................................................................ 58 The Principle of Displacement Chromatography................................. 59 Displacers for Biochromatography....................................................... 64 Polyelectrolytes .................................................................................... 73 Steric Mass Action Model.................................................................... 77 Systematic Displacer Design, Some Theoretical Considerations ................................................................................ 82 Conclusions......................................................................................... 84 5. Membrane Adsorbers for Decontamination and Leukocyte Removal ....................................................................... 87 Galya Tishchenko and Miroslav Bleha Introduction ........................................................................................ 87 Evaluation of the Adsorbing Efficiency of Interactive Membranes ................................................................ 88 Membranes for Depyrogenation (Endotoxin Removal) ....................... 90 Membranes for Removal of Bacteria and Viruses from Aqueous Solution ................................................................. 108 Membranes for Removal of Leukocytes from Blood Products ..................................................................... 110 Conclusions....................................................................................... 112 6. Stimulus Responsive Surfaces: Possible Implications for Biochromatography, Cell Detachment and Drug Delivery ........... 116 Igor Yu Galaev and Bo Mattiasson Stimulus-Responsive Polymers .......................................................... 117 Polymer-Grafted Surfaces .................................................................. 119 Temperature-Responsive Chromatography........................................ 120 Cell Detachment from Polymer-Grafted Surfaces .............................. 122 Controlling Porosity via Smart Polymers— The “Chemical Valve” ................................................................... 125 Polymer-Carrying Liposomes for Triggered Release/Drug Delivery ................................................................... 127 Conclusions....................................................................................... 129 7. Molecularly Imprinted Polymers: A New Dimension in Analytical Bioseparation ................................................................. 134 Oliver Brüggemann Introduction ...................................................................................... 134 The Principle of Molecular Imprinting.............................................. 134 MIP for Bioseparation ....................................................................... 141 Binding Assays Using MIP ................................................................ 149 Sensor Technology ............................................................................ 151 MIP to Assist Chemical Synthesis...................................................... 151 Conclusions....................................................................................... 154 Index .................................................................................................. 163

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