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Table of contents Proteomics in farm animals: going south 13 Pedro Rodrigues, David Eckersall and André Martinho de Almeida Part I - Invited plenary communications Mass spectrometry for the veterinary and farm animal world 19 Renata Soares, Catarina Franco, Elisabete Pires, Miguel Ventosa, Rui Palhinhas, Kamila Koci, André Martinho de Almeida and Ana Varela Coelho Protein synthesis pipelines for study of protein-protein interactions 21 Mangesh Bhide Dinosaurs and other farm animals 23 Jane Thomas-Oates Proteomics strategies to trace illegal growth-promoters in cattle 25 A. Urbani, C. Nebbia, M. Carletti, G. Gardini, D. Bertarelli, M. Ronci, L. Della Donna and P. Sacchetta Assessing fish quality in aquaculture: a proteomics approach 28 Pedro M. Rodrigues, Nadége Richard, Mahaut de Vareilles, Tomé S. Silva, Odete Cordeiro, Luis E.C. Conceição and Jorge Dias From meat to food: the proteomics assessment 31 Andrea Mozzarelli, Gianluca Paredi, Barbara Pioselli and Samanta Raboni Ruminant saliva: accessory proteins of homeostasis, olfaction and defence 35 Marcus Mau Proteomics of foes and friends adapted to milk environment: focus on Grampositive bovine mastitis bacteria and probiotics 40 Pekka Varmanen, Kerttu Koskenniemi, Pia Siljamäki, Tuula A. Nyman and Kirsi Savijoki 8 Farm animal proteomics Tick-borne diseases in cattle: applications of proteomics and the development of new generation vaccines 46 Isabel Marcelino, André Martinho de Almeida, Miguel Ventosa, Ludovic Pruneau, Damien F. Meyer, Dominique Martinez, Thierry Lefrançois, Nathalie Vachiéry and Ana Varela Coelho The applications of proteomics to animal models of leptospirosis: in vivo veritas 50 Jarlath E. Nally Part II - Proteomics and animal health Changes in the proteome of the H295R steroidogenesis model associated with exposure to the mycotoxin zearalenone and its metabolites, α- and β-zearalenol 55 Øyvind L. Busk, Doreen Ndossi, Caroline Frizzell, Steven Verhaegen, Silvio Uhlig, Gunnar Eriksen, Lisa Connolly, Erik Ropstad and Morten Sørlie Proteomic analysis of host responses to Salmonella enterica serovar Typhimurium infection in gut of naturally infected Iberian pigs 59 Cristina Arce, Angela Moreno and Juan Jose Garrido Biochemical and proteomic investigation of bovine nasal secretion 63 M. Faizal Ghazali, Nicholas Jonsson, Richard J.S. Burchmore and P. David Eckersall The behaviour of ceruloplasmin as an acute phase protein in obese and infected rabbits 67 T.M. Georgieva, T. Vlaykova, E. Dishlianova, Vl. Petrov and I. Penchev Georgiev Modifications of the acute phase protein haptoglobin in dairy cows 71 Susanne Häussler, Md. Mizanur Rahman, Andrea Henze, Florian Schweigert and Helga Sauerwein Rapid protein production pipeline in advanced inducible Leishmania tarentolae expression system 75 S. Hresko, P. Mlynarcik, L. Pulzova, E. Bencurova, R. Mucha, T. Csank, M. Madar, M. Cepkova and M. Bhide Brucellosis and proteomics: an approach in Albania 80 Jani Mavromati Saliva proteomics: tool for novel diagnosis for farm animal diseases from body fluids 84 Jacob Kuruvilla and Susana Cristobal Farm animal proteomics 9 Proteomics in biomarker detection and monitoring of pancreas disease (PD) in atlantic salmon (Salmo salar) 87 Mark Braceland, Ralph Bickerdike, David Cockerill, Richard Burchmore, William Weir and David Eckersall Results of proteomic mapping into milk infected by Streptococus agalactiae 91 Monika Johansson, Amaya Albalat, Aileen Stirling and William Mullen Adhesion of Francisella to endothelial cells is also mediated by OmpA:ICAM-1 interaction 94 R. Mucha, E. Bencurova, M. Cepkova, P. Mlynarcik, M. Madar, L. Pulzova, S. Hresko and M. Bhide Proteins overexpressed in ovine pulmonary adenocarcinoma 98 Anna Kycko and Michal Reichert Relevance of paraoxonase-1, platelet- activating factor acetylhydrolase and serum amyloid A in bovine mastitis 102 Romana Turk, Mislav Kovačić, Zlata Flegar Meštrić, Paola Roncada, Cristian Piras, Ante Svetina, Vesna Dobranić, Jelka Pleadin and Marko Samardžija Proteomic characterization of serum amyloid A protein in different porcine body fluids 106 L. Soler, J.J. Cerón, A. Gutiérrez, C. Lecchi, A. Scarafoni and F. Ceciliani Comparative exoproteomics of Staphylococcus epidermidis of human and bovine origin to identify bacterial factors involved in adaptation into bovine host 111 Kirsi Savijoki, Pia Siljamäki, Niina Lietzén, Pekka Varmanen, Matti Kankainen and Tuula A. Nyman Methodology of proteomic mapping into milk infected by Streptococus agalactiae 116 William Mullen, Amaya Albalat, Aileen Stirling, Justyna Siwy and Monika Johansson Part III - Proteomics in animal production Proteome analysis of muscles longissimus dorsi of Hungarian Merino and Tsigai sheep breeds 123 Levente Czeglédi, Krisztina Pohóczky, Gabriella Gulyás, Beáta Soltész and András Jávor Muscle proteomics profiles in sheep: the effect of breed and nutritional status 126 André M. de Almeida, Rui M.G. Palhinhas, Tanya Kilminster, Tim Scanlon, Johan Greeff, Chris Oldham, John Milton, Ana V. Coelho and Luís Alfaro Cardoso 10 Farm animal proteomics Cellular and molecular-large scale features of fetal perirenal and intermuscular adipose tissues in bovine 130 M. Bonnet, H. Taga, B. Picard and Y. Chilliard Vegetable based fish feed changes protein expression in muscle of rainbow trout (Oncorhynchus mykiss) 134 Flemming Jessen, Tune Wulff, Jeanett Bach Mikkelsen, Grethe Hyldig and Henrik Hauch Nielsen The proteomic insight of the Italian dry cured ham manufacturing 138 Gianluca Paredi, Samanta Raboni, Anna Pinna, Giovanna Saccani, Roberta Virgili and Andrea Mozzarelli IGF-1 free circulating protein and its gene-expression in linseed-rich diet quail 141 A. Karus, H. Tikk, A. Lember, V. Karus and M. Roasto A longitudinal proteomic approach to investigate liver metabolism in periparturient dairy cows with different body fat mobilization 145 Christine Schäff, Dirk Albrecht, Harald M. Hammon, Monika Röntgen, Cornelia C. Metges and Björn Kuhla The effect of colostrum intake on blood plasma proteomic profiles of newborn lambs 148 Lorenzo Enrique Hernández-Castellano, André Martinho de Almeida, Miguel Ventosa, Antonio José Morales-delaNuez, Ana Varela Coelho, Diego Martell Jaizme, Noemí Castro Navarro and Anastasio Argüello Henríquez Oxidative stress and acute phase response associated to changes in housing from pen to individual stalls in reproductive sows 152 Anna Marco-Ramell, Raquel Peña, Laura Arroyo, Raquel Pato, Yolanda Saco, Lorenzo Fraile and Anna Bassols Two-dimensional proteomics as a tool to evaluate nutritional effects in farmed fish 156 Nadège Richard, Paulo Gavaia, Mahaut de Vareilles, Tomé S. Silva, Odete Cordeiro, Pedro M. Rodrigues and Luis E.C. Conceição Serum proteomic analysis in bovine mastitis 159 Paola Roncada Cristian Piras, Luigi Bonizzi, Alessio Soggiu, Michele De Canio, Romana Turk, Mislav Kovačić, Marko Samardžija and Andrea Urbani From protein markers to phenotyping tools for evaluation of beef tenderness 165 Brigitte Picard, Bruno Meunier, Christiane Barboiron, Nicole Dunoyer, Nicolas Guillemin and Didier Micol Farm animal proteomics 11 Immunoreactive proteins of Mycobacterium avium subsp. paratuberculosis 169 C. Piras, A. Soggiu, L. Bonizzi, A. Urbani, V. Greco, G.F. Greppi, N. Arrigoni and P. Roncada Acute phase protein expression in different visceral and subcutaneous fat depots from clinically healthy dairy cows 173 Md. Mizanur Rahman, Susanne Häussler, Manfred Mielenz, Cristina Lecchi, Fabrizio Ceciliani and Helga Sauerwein The plasma proteome and acute phase proteins of broiler chickens with gait abnormalities 177 E.L. O’Reilly, R.J. Burchmore, V. Sandilands, N.H. Sparks, C. Walls and P.D. Eckersall Proteomic analysis of visceral and subcutaneous adipose tissue in goats 181 Laura Restelli, Dorte Thomassen, Marius Cosmin Codrea, Giovanni Savoini, Fabrizio Ceciliani and Emoke Bendixen Farm animal proteomics in toxicological studies: useful tool or waste of time? what to learn from rodent studies 185 T. Serchi, I. Miller, E. Rijntjes, J. Renaut, A.J. Murk, N.P. Evans, E. Ropstad, L. Hoffmann and A.C. Gutleb The role of SPEF2 in male fertility 188 Anu Sironen, Jeanette Hansen, Bo Thomsen, Magnus Andersson, Johanna Vilkki, Jorma Toppari and Noora Kotaja Proteomic analysis of cryoconserved bull sperm to enhance ERCR classification scores of fertility 190 Alessio Soggiu, Cristian Piras, Luigi Bonizzi, Alessandro Gaviraghi, Andrea Galli, Hany Ahmed, Paolo Sacchetta, Andrea Urbani and Paola Roncada Proteomics biomarkers for pork quality 194 Marinus F.W. te Pas, Mariusz Pierzchala, Els Keuning, Ron E. Crump, Sjef J.A. Boeren, Maria Hortos, Marina Gisbert, Alejandro Diestre and Leo Kruijt Proteomics as a tool to understand fish stress in aquaculture 198 Tomé S. Silva, Pedro M. Rodrigues, Elisabete Matos, Tune Wulff, Odete D. Cordeiro, Ricardo N. Alves, Nadège Richard, Mahaut de Vareilles, Flemming Jessen, Jorge P. Dias and Luís E.C. Conceição 12 Farm animal proteomics A peptidomic approach to biomarker discovery for bovine mastitis 202 Rozaihan Mansor, William Mullen, David C Barrett, Andrew Biggs Amaya Albalat, Justyna Siwy, Harald Mischak and P. David Eckersall Acknowledgements 207 Major sponsors 207 Organizing support 208 Individual contributions 208

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